Simultaneous Detection and Quantification of Four Sulfonamides in Buffalo Meat using RP-HPLC

S.  Kalpana; Ch.Umamaheswara  Rao; M.  Muthukumar

doi:10.48165/jms.2022.1706

Authors

S. Kalpana Chemical Residues Laboratory, ICAR-National Research Centre on Meat, Chengicherla, Hyderabad- 500092, India
Ch.Umamaheswara Rao Chemical Residues Laboratory, ICAR-National Research Centre on Meat, Chengicherla, Hyderabad- 500092, India
M. Muthukumar Chemical Residues Laboratory, ICAR-National Research Centre on Meat, Chengicherla, Hyderabad- 500092, India

DOI:

https://doi.org/10.48165/jms.2022.1706

Keywords:

RP-HPLC, Buffalo meat, Tissue residues, Sulfonamides, liquid-liquid extraction

Abstract

A multi-residue analysis of four sulfonamides viz., sulfadiazine, sulfadoxine, sulfamethazine, sulfamethoxazole in buffalo meat has been proposed using HPLC- UV detection method. Samples were extracted by liquid-liquid extraction using acetonitrile, n-hexane solution and the analysis was carried out using a reverse phase HPLC using UV detector. The chromatographic separation was achieved using mobile phase consisting of glacial acetic acid in acetonitrile, methanol and water (85:10:5, v/v) in an isocratic elution mode. The volume of injection and flow rate of the mobile phase were 40 µl and 1.5 ml/min respectively. UV detector was operated at a wavelength 254 nm. Specificity, linearity, recovery percentage, intra-day variation, limit of detection (LOD), limit of quantification (LOQ) were evaluated in buffalo meat matrix at drug concentrations ranging from 10-500µg/Kg. Extraction recoveries of sulfonamides viz., sulfadiazine, sulfadoxine, sulfamethazine, sulfamethoxazole were ranged to be 72-77, 79-83, 85-89 and 82-86% respectively. The LOD for Sulfonamides viz., sulfadiazine, sulfadoxine, sulfamethazine, sulfamethoxazole were 6.06, 7.63, 6.79 and 10.68 µg/Kg and the LOQ were 18.37, 23.12, 20.57 and 32.37 µg/Kg, respectively. The proposed RP-HPLC method is quite adequate for routine monitoring of residual sulfonamides in buffalo meat below Codex MRL (100 µg/Kg).

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