Effect of Guava Leaves Powder on the Oxidative and Microbial Stability of Chicken Meat Nuggets during Refrigerated (4±1°C) Storage
DOI:
https://doi.org/10.48165/jms.2026.21.01.5Keywords:
Guava leaf powder, antioxidants, antimicrobial agents, chicken meat nuggets, storage stability.Abstract
The present study was carried out to evaluate the efficacy of different levels (0.5 and 1 per cent) of guava leaf powder (GLP) (Psidium guajava L) with synthetic antioxidant ie) butylated hydroxy toluene (BHT) at 0.01 % was studied on physico-chemical, microbial and sensory quality characteristics of chicken meat nuggets under aerobic packaging conditions during refrigerated storage (4±1°C). The chicken nuggets added with 1 % GLP had significantly (P < 0.05) lower 2-thiobarbituric acid reactive substance (TBARS) values and free fatty acid (FFA) content compared to control nuggets and other samples during refrigerated storage (4±1°C). A significant (P<0.05) lower total plate count and total psychrophilic count were recorded in chicken nuggets added with GLP. Addition of 1 % GLP significantly (P<0.05) reduced the coliform count and yeast and mould counts than control and BHT added chicken nuggets. 1 per cent GLP added chicken meat nuggets rated significantly (P<0.05) superior scores of colour, flavor, tenderness, juiciness and overall acceptability than control, BHT and 0.5 % GLP added chicken meat nuggets. Based on the obtained results, it was concluded that 1 % GLP had excellent antioxidant and antimicrobial properties compared to control and BHT added chicken meat nuggets during refrigerated storage under aerobic conditions and application of GLP as natural antioxidant and anti microbial agent in chicken meat nuggets which extends shelf life up to 20 days in refrigerated storage without any quality deterioration.
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References
Andres AI, Petron MJ, Adamez JD, Lopez M, Timon ML (2017). Food by-products as potential antioxidant and antimicrobial additives in chill stored raw lamb patties. Meat Science 129: 62–70.
AOAC (2002). Official Method of Analysis. Rev.1. 17th edn., Association of Official Analytical Chemists Inc, Arlington VA, pp. 1-23.
Banerjee R, Verma AK, Das AK, Rajkumar V, Shewalkar AA, Narkhede HP (2012). Antioxidant effects of broccoli powder extract in goat meat nuggets. Meat Science 91: 179–184.
Bhaskar Reddy GV, Sen AR, Pramod N Nair, Reddy KS, Reddy KK and Kondaiah N (2013). Effects of grape seed extract on the oxidative and microbial stability of restructured mutton slices. Meat Science 95: 288–294.
Bhaskar Reddy GV, Sen A, Indumathi J, Nagamallika E, Rambabu D, Rao P (2018). Influence of natural antioxidants on storage stability of vacuum packed pork patties during refrigerated storage. International Journal of Livestock Research 8(4): 219-228.
Brannan RG (2009). Effect of grape seed extract on descriptive sensory analysis of ground chicken during refrigerated storage. Meat Science 81:589-595.
Brannan RG, Mah E (2007). Grape seed extract inhibits lipid oxidation in muscles from different species during refrigerated and frozen storage and oxidation catalyzed by peroxynirite and iron/ascorbate in a pyrogallol red model system. Meat Science 77(4): 540-546.
Cagdas E, Kumcuoglu S (2015). Effect of grape seed powder on oxidative stability of precooked chicken nuggets during frozen storage. Journal of Food Science and Technology 52: 2918–2925.
Chiari-Andreo BG, Trovatti E, Marto J, Almeida-Cincotto MGJ, Melero A, Correa MA, Chiavacci LA, Ribeiro H, Garrigues T, Isaac VLB (2017). Guava: phytochemical composition of a potential source of antioxidants for cosmetic and/or dermatological applications. Brazilian Journal of Pharmaceutical Science 53:16141.
Correa LC, Santos CAF, Vianello F, Lima GPP (2011). Antioxidant content in guava (Psidium guajava) and araca (Psidium spp.) germplasm from different Brazilian regions. Plant Genetics Resources 9:384-391.
Das M, Goswami S (2019). Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: Role of phytochemicals. International Journal of Health Science and Research 9: 39.
Delorino SB, Ogalesco ML, Rebadulla KR, Rongcales MTA, Salubre JIA, Talacay MKS, Tuballas ZB (2020). Wound healing efficacy of guava leaf extract. Journal of Pharmaceutical Research International 32 (41): 27-35.
Dewage DDDH, Samarakoon SMGK, Karunarathne SHS, Rajapakse CSK (2023). Psidium guajava L. (Common Guava) peel, pulp and leaves as natural sources of antioxidants, antimicrobials and photoprotective agents for development of sun protection cosmeceuticals Asian Journal of Chemistry 35(1): 89–94.
Estevez M, Xiong Y (2019). Intake of oxidized proteins and amino acids and causative oxidative stress and disease: Recent scientific evidences and hypotheses. Journal of Food Science 84: 387–396.
Ferreira VCS, Morcuende D, Madruga MS, Silva FAP, Estevez M (2018). Role of protein oxidation in the nutritional loss and texture changes in ready-to-eat chicken patties. International Journal of Food Science and Technology 53:1518–1526.
Gaber NB, El-Dahy SI, Shalaby EA (2023). Comparison of ABTS, DPPH, permanganate, and methylene blue assays for determining antioxidant potential of successive extracts from pomegranate and guava residues. Biomass Conversion and Biorefinery 13:4011– 4020.
Garcia‐villegas A, Rojas‐garcia A, Villegasaguilar MDC, Fernandez‐moreno P, Fernandez‐ochoa A, Cadiz‐gurrea MDIL, Arraez‐roman D, Segura‐carretero A (2022). Cosmeceutical potential of major tropical and subtropical fruit by-products for a sustainable revalorization. Antioxidants 11(2): 1–37.
Growther L, Sukirtha K (2018). Phyto chemical analysis and antimicrobial properties of leaves and Psidium guajava bark extracts. Asian Journal of Pharmacy and Pharmacology 4(3):318-323.
Gurusamy D, Henning AN, Yamamoto TN, Yu Z, Zacharakis N, Krishna S (2020). Multi-phenotype CRISPR-Cas9 screen identifies p38 kinase as a target for adoptive immunotherapies. Cancer cell 37 (6):818-833.
ICMSF (1983). International commission of microbiological specifications for foods. In R. P. Elliott, D. S. Clark, K. H. Lewis, H. Lundbeck, J. C. Olson, & Simonsen (Eds.), Vol.1, Toronto, Canada: University of Toronto Press.
Jiang J, Xiong YL (2016). Natural antioxidants as food and feed additives to promote health benefits and quality of meat products: A review. Meat Science 120:107–117.
Jimenez-Escrig A, Rincon M, Pulido R, Saura-Calixto F (2001). Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber. Journal of Agricultural Food Chemistry 49:5489-5493.
Keeton JT (1983). Effect of fat and NaCl/phosphate levels on the chemical and sensory properties of pork patties. Journal of Food Science 48: 878-881.
Kim DO, Jeong SW, Lee CY (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry 81: 321–326.
Koniecko EK (1979). In: Handbook for meat chemists. Ch.6, Avery Publishing group Inc., Wayne, New Jersey, USA. pp. 68-69.
Kumar M, Tomar M, Amarowicz R, Saurabh V, Nair MS, Maheshwari C (2021). Guava (Psidium guajava L.) leaves: Nutritional composition, phyto chemical profile, and health-promoting bioactivities. Foods 10:752.
Liu X, Yan X, Bi J, Liu J, Zhou M, Wu X, Chen Q (2018). Determination of phenolic compounds and antioxidant activities from peel, flesh, seed of guava (Psidium guajava L.) Electrophoresis 39(13): 1654–1662.
Mahapatra GS, Biswas S, Patra G, Vidyarthi AK (2019). Enhancement of storage stability, textural profile, physico-chemical parameters and sensory attributes of chevon meatballs by guava powder incorporation. International Journal of Bio-resource and Stress Management 10(4):346-351.
Mercadante AZ, Steck, Pfander H (1999). Carotenoids from guava (Psidium guajava L): isolation and structure elucidation. Journal of Agricultural Food Chemistry 47: 145-151.
Nahar N, Rahman S, Mosihuzzaman M (1990). Analysis of carbohydrates in seven edible fruits of Bangladesh. Journal of Science of Food and Agriculture 51:185-192.
Naseer S, Hussain S, Naeem N, Pervaiz M, Rahman M (2018). The phytochemistry and medicinal value of Psidium guajava (guava). Clinical Phytoscience 4: 32.
Shabbir H, Kausar T, Noreen S, Rehman HU, Hussain A, Huang Q, Gani A, Su S, Nawaz A (2020). In Vivo Screening and Antidiabetic Potential of Polyphenol Extracts from Guava Pulp, Seeds and Leaves. Animals 10(9): 1714:1-14.
Singh RP, Murthy K NC, Jayaprakasha GK (2002). Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of Agricultural and Food Chemistry 50: 81–86.
Singleton VL Orthofer R, Lamuela-Raventos RM (1999). Analisys of total phenols and other oxidation sobstrates and antioxidants by means of Folin Ciocalteau reagent. Methods Enzymology 299: 152–178.
Soliman FM, Fathy MM, Salama MM, Saber FR (2016). Comparative study of the volatile oil content and antimicrobial activity of Psidium guajava L. and Psidium cattleianum sabine leaves. Bull. Fac. Pharm. Cairo Univ. 54:219–225.
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D (2006). Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composting and Analysis 19:669-675.
Tran TTT, Ton NMN, Nguyen TT, Le VVM, Sajeev D, Schilling MW, Dinh TTN (2020). Application of natural antioxidant extract from guava leaves (Psidium guajava L.) in fresh pork sausage. Meat Science 165:108106.
Verma AK, V. Rajkumar, Rituparna Banerjee1, S. Biswas and Arun K Das (2013). Guava (Psidium guajava L.) powder as an anti oxidant dietary fibre in sheep meat nuggets. Asian Australasian Journal of Animal Science 26 (6): 886-895.
Wang L, Bei, Q, Wu Y, Liao W, Wu Z (2017). Characterization of soluble and insoluble-bound polyphenols from Psidium guajava L. leaves co-fermented with Monascus anka and Bacillus sp. and their bio-activities. Journal of Functional Foods 32:149–159.
Witte VC, Krause GF and Bailey ME (1970). A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal of Food Science 35: 82-585.
Yousaf AA, Abbasi1KS, Ahmad1 A, Hassan I, Sohail A, Qayyum A, Akram MA (2021). Physico-chemical and nutraceutical characterization of selected indigenous guava Psidium guajava L.) cultivars. Food Science and Technology, Campinas 41(1): 47-58.
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