HPTLC Finger Printing Profile Of Drynaria Quercifolia (L.) J. Sm. With Biomarker Naringin
Keywords:
HPTLC, Drynaria quercifolia, biomarker,, Ayurveda, naringin, inflammationAbstract
Drynaria quercifolia (L.) J. Sm. is a common and safe medicinal fern of tropical climate, used by Ayurvedic physicians of Kerala and tribal peoples in different parts of the country for various ailments with inflammation. But, to get a desired therapeutic effect the drug must be genuine with good quality. Authentication and quality of plants can be accurately done by doing HPTLC fingerprints with biomarker. Development of HPTLC finger printing profile of aqueous extract of rhizome of the plant with biomarker naringin was the aim of this study. Precoated silica gel 60 F254 was the stationary phase and ethyl acetate, formic acid, acetic acid, water in the ratio -1.5:1.1:1.1:0.1 was the mobile phase used for this purpose. After derivatization with methanolic sulphuric-10%, TLC plates were viewed at UV 366 nm and in visible light and densitometric profile were developed at 590 nm. At 590 nm, chromatogram of the aqueous extract of the drug showed 4 peaks and the 3rd peak was found to be homologous to the chromatogram of naringin. Thus the study revealed the identification as well as genuineness of the plant and has shown the potency of the drug as an anti-inflammatory agent.
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References
G Prasanna, R Anuradha. A Comprehensive Review on Phytopharmacological Activities of Drynaria quercifolia L. Int. J. Pharmacogn. Phytochem., 2016;8(8): 1304-1313. Available from:https://www.researchgate.net/publication/3
_A_comprehensive_review_on_phyto pharmacological_activities_of_drynaria_quercif olia_L
Janarthanan L, Pharmacognostical standardization and phytochemical profile of rhizomes of drynaria quercifolia (Linn) J Smith. Eur J Pharm Sci., 2016; 3(8):278–87. Available from:
https://www.researchgate.net/publication/30869 9562_Pharmacognostical_Standardization_And
_Phytochemical_Profile_of_rhizomes_of_Dryna ria_quercifolia_Linn_J_Smith
Kumar K N, Comprehensive Pharmacognostical Profile of Drynaria quercifolia (L.) J. Sm. IJAM., 11(4):672–8. Available from DOI: https://doi.org/10.47552/ijam.v11i4.1657
Hendrik A.V.Hortus Malabaricus Vol. 12. Prof K S Manilal (ed.). Thiruvanathapuram; Department of Publications University of Kerala: 2008.
Sreeman N. Chikitsamanajri. 7th ed., Alappuzha; Vidhyarambham publishers: 2005.
Sreeman N. Yogamrutham. 3rd ed., Alappuzha; Vidhyarambham publications: 2004.
Ramdas N. Kale, High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves. J. Pharm. Res. Int., 2020; 32(48): 8-15. Available from DOI: 10.9734/jpri/2020/v32i4831120
Daya L. C HPTLC finger print profile and isolation of marker compound of Ruellia tuberosa. Chromatogr. Res. Int., 2012. Available from DOI: https://doi.org/10.1155/2012/180103
Batool S, Antidermatophytic activity of Drynaria quercofolia (L.) J. Smith. Jundishapur J Microbiol., 2009; 2(1): 25–30. Available from: https://www.researchgate.net/publication/43297
_Antidermatophytic_activity_of_Drynaria_q uercifolia_L_J_Smith
Daya L. Chothani, M. B. Patel, S. H. Mishra. HPTLC finger print profile and isolation of marker compound of Ruellia tuberosa. Chromatogr. Res. Int., 2012. Available from DOI: https://doi.org/10.1155/2012/180103
Rui C,Therapeutic potential of naringin: an overview. Pharm. Biol., 2016; 54 (12): 3203- 3210. Available from DOI: https://doi.org/10.1080/13880209.2016.1216131
