Studies on cryopreservation of cauda epididymal spermatozoa buffaloes

Authors

  • HIRON M HARSHAN Animal Reproduction Division --~\.. .1,i- *\... _,I~ , Indian Veterinary Research Institute, lzatnagar - 243 122 (UP)
  • L P SINGH

Keywords:

Buffalo, cauda epididymal spermatozoa, cryopreservation

Abstract

The effect of cryopreservation on the viability, motility, acrosomal integrity, cervical mucus penetration ability and
response to hypo-osmotic solution was studied on samples of cauda epididymal semen collected from 12 mature buffalo bulls
slaughtered at the local slaughter house. Though a significant reduction (P<0.05) in all the parameters was observed after
cryopreservation it was concluded that successful cryopreservation of the cauda epididymal semen can be done. The viability,
motility, acrosomal integrity, cervical mucus penetration ability and response to hypo-osmotic solution were reduced from
83.92±0.80%, 72.75±0.91%, 80.42±0.82%, 21.17±0.75 mm and 72.00±0.93%to 68.67±0.94%,51.58±1.18%, 66.83±1.05%,
17.67±0.84 mm and 59.67±0.85%, respectively .

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Published

2023-04-12

Issue

Vol. 27 No. 1 (2006): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

HARSHAN, H.M., & SINGH, L.P. (2023). Studies on cryopreservation of cauda epididymal spermatozoa buffaloes. The Indian Journal of Animal Reproduction, 27(1), 47–50. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/6589