Studies on the microbial load of cryopreserved Murrah semen
Keywords:
Buffalo, semen, microbial load, standard plate countAbstract
The objective of the present study was to detennine microbial load in frozen semen of three Murrah bulls using ten ejaculates
from each bulls. The average bacterial load determined using pour plate method in the frozen semen of the three bulls was 79.00±0.76,
7.00±0.91 and 60.00±0.87, respectively with an overall mean of 48.60±0.73 colony forming units (CFU) m1·1• The SPC differed
significantly (P< 0.01) between the three bulls. All the semen samples were found to be negative for fungus, mucor and yeast.