Studies on certain attributes of buffalo follicular fluid

Abstract

The aim of the present investigation was to determine average yield of follicular fluid, total protein, estradiol and
progesterone profile in the follicular fluid of buffalo ovaries. The follicular fluid was collected by aspirating the visible surface
follicles. The average yield of buffalo follicular fluid (buFF) per ovary was 0.45±0.03 ml which was higher during the months between
November to March (0.54±0.02 ml) than April to September (0.38±0.02 ml). The mean concentration of total protein in pooled
buFF samples was 4.86±0.41 g% (ranged between 3.0 to 6.7 g%). The mean concentration of estradiol and progesterone in pooled
buFF samples were 3044.99±31.85 pg/ml and 74.31±2.94 ng/rnl, respectively.