Effect of three dose levels of buffalo follicular fluid on estrus induction response in anoestrous goats

Abstract

The present study was designed to examine the effect of three dose levels of buffalo follicular fluid on estrus induction
response in anoestrous goats. A total of 40 aparently healthy and adult anoestrous does were equally divided into 4 groups i.e. G1,
G2, G3 and G4• Ten does in each group were treated with charcoal (@ 5 mg/ml) extracted buffalo follicular fluid @ 12 ml (G1), 18
ml (G2) and 24 ml (G,) through subcutaneous route twice daily for 3 consequitive days. Ten does were kept as. untreated control
(G4). All the does following treatment were subjected to estrus detection at 12 hours interval. Total 16 (53.3%) out of 30 does
showed estrus following buFF treatment. The proportion of does in estrus was higher (P > 0.05) in 18 ml (G2) as compared to 24
ml (G,) (50%; 5/10) and 12 ml (G) (40%, 4/10) groups, respectively. None exhibited estrus in control group (GJ. The mean
interval between last buFF treatment to onset of estrus were 6.3:t:1.93, 6.4:t:1.17 and 7.6:t:0.87 days and developed estrus was
18.0±3.46, 41.l:t:10.43 and 16.8:t:7.91 h in GI' G2 and G3 groups, respectively. The difference in mean duration of time taken for
onset of estrus among the groups was not significant (P > 0.05). The results demonstrated the beneficial effect of charcoal treated
buffalo follicular fluid for induction of estrus in anoestrus goats. Total dose of 18 ml buFF was found to be better for induction of
estrus in anoestrus goats.