Effect of glutathione on leakage of various enzymes from buck semen during freezing

Authors

  • M P SINHA Department of Gynaecology, Ranchi Veterinary College, Birsa Agricultural University, Kanke, Ranchi
  • A K SINHA
  • R L PRASAD

Keywords:

Goat, glutathione, acrosin, hyaluronidase, frozen semen

Abstract

Semen samples from 12 bucks were extended in Tris dilutor with 2 and 5 mM of glutathione. The activities of acrosome,
hyaluronidase, AKP, AST, ALT and LDH were assayed in equilibrated and frozen-thawed semen samples. Significantly (P < 0.01)
higher intracellular activity of acrosin was recorded in semen samples extended with 5 mM of glutathione. Effect of glutathione on
acrosin activity was significant at both the pre and post-freeze stage. Addition of 5 mM glutathione also decreased significantly the
release of hyaluronidase, AKP, AST, ALT and LDH enzymes from the sperm cell in the medium.

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Published

2023-03-29

Issue

Vol. 25 No. 2 (2004): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

SINHA, M.P., SINHA, A.K., & PRASAD, R.L. (2023). Effect of glutathione on leakage of various enzymes from buck semen during freezing. The Indian Journal of Animal Reproduction, 25(2), 113–115. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/6318