Effect of different capacitating agents on in vitro fertilization of goat follicular oocytes

Abstract

The present investigation is an attempt to establish optimal culture conditions for in vitro fertilization (!VF) of goat
oocytes. Ovaries were obtained from a slaughter house and cumulus oocytes complexes (COCs) were washed three times in TLHEPES
medium and were matured for 24 h in TCM-199 (M-199) media supplemented with 10% fetal calf serum (FCS) and
hormones (I mg/ml 17/3 estradiol, E2; 0.5 mg/ml ml follicle stimulating hormones (FSH) and 5.0 mg/ml leutinizing hormone, LH).
After 24 hrs. of incubation oocytes were inseminated with percoll separated epididymal spermatozoa in Brackett and Oliphant (BO)
or Talp-fertilization medium and treated with either Ca++ ionophore (A-23187) (0.1, 0.2 and 0.5 mM/ml + 5mM caffeine / L ) or
heparin (0,1,10 and 100 mg/ml)+ 5mM caffeine IL or heparin (0,1,10 and I00mg/ml) alone in Talp-fertilization medium.