Standardization of hypo-osmotic solutions to study the functional integrity of post thaw bull spermatozoa

Authors

  • AJEET SINGH Punjab Agricultural University, Ludhiana - 141 004 (Punjab)
  • JAGIR SINGH
  • J S MATHAROO
  • PRAHLAD SINGH

Keywords:

HOST, functional integrity, post thaw semen, curled tail spermatozoa, bull

Abstract

Functional integrity of sperm plasma membrane was studied in post thaw bull semen using Hypo-osmotic swelling test
(HOST). Frozen semen after thawing was subjected to hypo-osmotic swel!ing test (HOST) in hypo-osmotic solutions of osmolarity
75, 100 and 150 mOsmol/L. The overall average tail curling with 75, 100 and 150 mOsmol/L were recorded 17.55±8.28, 46.46±11.97
and 34.27±7.97%, respectively. Maximum tail curling was observed with JOO mOsmol/L solution which was significantly (P < 0.05)
higher than the 75 ahd 150 mOsmol/L solutio.

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Published

2023-03-30

Issue

Vol. 25 No. 1 (2004): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

SINGH, A., SINGH, J., MATHAROO, J.S., & SINGH, P. (2023). Standardization of hypo-osmotic solutions to study the functional integrity of post thaw bull spermatozoa. The Indian Journal of Animal Reproduction, 25(1), 48–49. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/6285