Effect of live sperm count per inseminate on pregnancy rate in buffaloes
Keywords:
Buffalo bull, Number of spermatozoa, Pregnancy rate.Abstract
Semen from 1 0 MUITah buffalo bulls was split-frozen in 0.5 ml French straws to get mean concentrations
of22.6, 17.3, l l.0and 8.Sx 106(Cl, C2, C3 & C4, respectively)spermatozoa per dose. A total of1941 buffaloes were
inseminated at 20 AI centres and pregnancy rates were 59.4, 58.1, 66.7 and 52.4 per cent for CI, C2, C3 and C4
respectively. Pregnancy rates differed significantly among bulls and among technicians. After correcting for the
variations due to bulls as well as technicians, the pregnancy rates were not affected by the sperm concentration
employed. It is concluded that the reduced sperm concentration up to 11 x l 06 spermatozoa or even lower
concentration (8.5 million) per inseminate does not affect the overall fertility of buffalo bull semen, though individual
differences exist between bulls regarding maintenance of fertility at lower sperm concentration per inseminate.