Cytological changes during certain reproductive conditions in in-vitro uterine flushing in buffaloes (Bubalus bubalis)

Abstract

Cytological changes during follicular, luteal phase, inactive ovarian stage and infected uterine flushings
in-vitro were studied in buffalo uteri (n-54) collected from local abattoir. The overall total nucleated and epithelial
cell count was significantly (P<O.O I) higher in infected uteri compared to follicular phase and inactive ovarian
stage. however, differance was non-significant during luteal phase but these cells varied significantly (P<O.O J)
between late-luteal phase and infected uteri. Absolute lymphocyte counts, and lymphocyte percentages varied
non-significantly between two uterine horns as well as during different stages of uterine flushings, however,
absolute neutrophil count and neutrophil percentage showed significant (P<O.O I) increase in the infected uteri as
compared to other stages. Cytological studies cou!d be used as an adjunct to diagnose sub-clinical uterine
infections. However, in-vivo study on large number of bovines, particularly in buffaloes is warrante.