Improvement of buffalo bull semen quality through sephadex filtration and subsequent assessment through polyacrylamide gel column migration

Authors

  • SATWINDER SINGH Department of Animal Reproduction. Gynaecology & Obstetrics. College of Veterinary Sciences. Punjab Agricultral University, Ludhiana - 141 00 I
  • G R PANGAONKAR
  • K C CHOUDHARY
  • H K VERMA

Keywords:

Buffalo semen quality, Sephadex filtration, freezing of semen, sperm motility

Abstract

To study the effect of sephadex (G 15- 120) filtration in improving the buffalo bull semen quality, 20
ejaculates from four bulls exhibiting poor freezability were taken. Sephadex filtered Tris-yolk extended semen
exhibited significant(P < 0.05) improvement in sperm motility (63.75 ± 0.99 vs 71 .75 ± 0. 81 percent), live sperm
count ( 75.15 ± 0.65 vs 83.00 ± 0.70 percent) and spermatozoa! penetrability through PAG column (20.35 ± 0.26 vs
22.43 :t 0.32 mm/20 min.) as compared to unfiltered semen. Subsequently sephadex filtration improved the freezability
of .semen by increasing (p <0.05) its post thaw motility(29.50 ± 0.85 to 42.75 ± 1.02 percent), livability(4 l.60± 0.88
to 52.90 ± 1.30 percent) and percent spermatozoa! penetrability through polyacrylamide gel column ( 17.71 ± 0.07 to
19.41 ::::0.l6mm/20min.).

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Published

2023-02-12

Issue

Vol. 23 No. 2 (2002): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

SINGH, S., PANGAONKAR, G.R., CHOUDHARY, K.C., & VERMA, H.K. (2023). Improvement of buffalo bull semen quality through sephadex filtration and subsequent assessment through polyacrylamide gel column migration. The Indian Journal of Animal Reproduction, 23(2), 120–122. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/5943