Studies on Seminal Enzyme (GOT, GPT and LDH) Profile and its Relationship with Acrosomal Integrity in Surti Buffalo Bull Semen

Authors

  • RAJEEV SHARMA Department of Veterinary Obstetrics and Gynaecology, College of Veterinary and Animal Science, Rajasthan Agricultural University, Bikaner (Rajasthan), India-334001
  • P K PAREEK
  • S P TAILOR
  • G N PUROHIT

Keywords:

Acrosome, GOT, semen, seminal enzymes

Abstract

Six ejaculates each from 5 Surti buffalo bulls were cryopreserved and studied before and after
cryopreservation and at½, 1 and 2 hours post thawing to evaluate the extent of damage of spermatozoa.
The sperm motility and percent live sperms and percent normal acrosomes showed a decline after
cryopreservation. The seminal enzymes GOT, GPT and LDH increased in the seminal plasma to
significanly high level (P<0.01) post freezing and thawing and at different times of post thaw incubation
indicating a leakage from the spermatozoa. It was concluded that freezing and thawing of semen
causes cryoinjury to the sperms, decrease in the percentage of intct acrosomes and leakage of semen
enzymes.

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Published

2003-06-30

Issue

Vol. 24 No. 1 (2003): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

SHARMA, R., PAREEK, P.K., TAILOR, S.P., & PUROHIT, G.N. (2003). Studies on Seminal Enzyme (GOT, GPT and LDH) Profile and its Relationship with Acrosomal Integrity in Surti Buffalo Bull Semen. The Indian Journal of Animal Reproduction, 24(1), 75–76. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/5915