Effect of equilibration period on quality of frozen semen in Beetal x Assam local crossbred goats
Keywords:
Equilibration period, frozen semen, sperm motility, intact acrosome, crossbred goatAbstract
A total of 36 pooled ejaculates collected from four adult crossbred goats (Beetal x Assam Local
goats) were used to study the effect of three equilibration periods on quality of frozen semen. The
semen extended in Tris-egg yolk-citric acid-fructose-glycerol extender was frozen in French medium
straws in liquid nitrogen vapour. The mean per cent sperm motility and intact sperm acrosome after
freezing were significantly higher at 5 hours (54.58±1.30, 81.33±0.17) than at 3 (48.75±1.39, 80.58±0.25)
and 7 (51.67±0.71, 79.21±0.31) hours of equilibration periods.