Effect of prostaglandin F2alpha, oxytocin and butylated hydroxytoluene on enzyme activities of crossbred (HF x Hariana) bull semen

Abstract

Semen samples were collected from two crossbred bulls (HF x Hariana) and diluted in tris with
three combinations of additives i.e., prostaglandin Fp, oxytocin and butylated hydroxytoluene (2mM/
ml) and frozen in liquid nitrogen. The enzyme activities (GOT, GPT, ACP and AKP) were evaluated
at prefreeze and postfreeze stage. Except GPT the other enzymes showed no significant difference
between treated and control groups at prefreeze stage. The value of GOT and GPT differed significantly
between treatment and control groups, whereas, there was no significant difference in ACP and AKP
at postfreeze stage. The enzymatic activity in the post-thaw semen treated with these additives revealed
a lower leakage of enzyme than control.