Disposition of enrofloxacin administered intrauterine in buffaloes with endometritis

Abstract

The present study was aimed at estimating extent of enrofloxacin absorption across the uterus
after its intrauterine administration. Enrofloxacin (3 mg/kg B.wt.) was administered (I/U) to six
buffaloes with history of endometritis and blood samples before and 1,2,3,4 and 5 hours after
administration were collected. The enrofloxacin levels were detected by employing microbiological
assay technique, using Bacillus subtilis ATCC-6633 as test organisms. Disposition studies showed
that detectable levels (0.08 mcg/ ml) of enrofloxacin in blood were achieved one hour after intrauterine
administration and persisted upto 4 hours. Therapeutically effective levels were achieved upto 2 to 3
hrs. It was concluded that therapeutically effective enrofloxacin levels could not be achieved in blood
and uterine tissues after intrauterine infusion with enrofloxacin 1500 mg.