Effect of prostaglandin F2alpha, oxytocin and butylated hydroxytoluene on freezability of crossbred bull spermatozoa

Abstract

Semen samples were collected from two crossbred bulls (HF x Hariana) and diluted in Tris with three
combinations of ad~tives i.e. prostaglandin F 2 a, oxytocin and butylated hydroxytoluene and frozen in liquid
nitrogen. The sperm motility, livability and infact acrosome percentage were evaluated at pre-freeze and postfreeze
stages. Except prostaglandin F 2 a, the other additives showed no significant difference in motility after
dilution vs control. At pre-freeze stage, sperm motility differed significantly between treated and control groups.
At post-freeze stage, sperm motility and livability differed significantly between treatment and control groups.
Among the additives PGF 2a performed better than oxytocin and BHT in maintaining higher motility, livability
and acrosomal integrity in post-thaw semen.