Effect of glutathione supplementation during sperm preparation and in vitro fertilization on ovine embryo development

Authors

  • S RANGASAMY Assisted Reproductive Technology Laboratory, Department of Animal Reproduction, Gynaecology and Obstetrics, Madras Veterinary College, Chennai - 600 007.
  • K KULASEKAR
  • S BALASUBRAMANIAN
  • G DHINAKAR RAJ

Keywords:

Ovine, Glutathione, In vitro fertilization, Embryo

Abstract

The present study was conducted to investigate the effect of glutathione (GSH) supplementation during
sperm preparation media (SPM) and in vitro fertilization (IVF) media on ovine embryo development. Six
independent trials with a total of273 oocytes were carried out with GSH supplementation at 0.3 mg/ ml during
SPM and IVF, 263 oocytes without supplementation formed the control. The mean cleavage rates were 48.46 ±
0. l 5 and 46.68 ± 0.12 per cent (P<0.0 I) in the treatment and control group, respectively. The percentage of
morula (mean ± SE) that developed in treatment and control group were 24.58 ± 0.32 and 19.80 ± 0.24,
respectively. The percentage of embryo that developed to the morula stage were significantly higher (P<0.0 l) in
treatment group. GSH supplementation dijring SPM and IVF improved the cleavage and morula development.

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Published

2023-02-02

Issue

Vol. 29 No. 1 (2008): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

RANGASAMY, S., KULASEKAR, K., BALASUBRAMANIAN, S., & RAJ, G.D. (2023). Effect of glutathione supplementation during sperm preparation and in vitro fertilization on ovine embryo development. The Indian Journal of Animal Reproduction, 29(1), 89–91. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/5418