Relation of seminal plasma hormonal levels with quality of frozen semen in cattle

Abstract

Levels of seminal plasma Testosterone, Tri-iodo-thyronine {T3) and Thyroxin {T4) in the fresh ejaculatet
were estimated aild its correlation with post thaw seminal quality was found out. The levels of these honnones
were significantly_ {p<0.01) higher in HF semen than in crossbred and Murrah semen. These hormones w~re
positively correlated with sperm motility, volume, total sperm concentration and percent live·sperm. A significant
positive correlation with sperm penetration distance (SPD), perc~nt sperm responding to hypo-osmotic swelling
test (HOS) in fresh -and post-thawed semen with these hormones was also recorded. A positive correlation of
SPD and HOS positive sperm with testosterone, T3 and T4; both in fresh and post-thawed semen proves their
important role in maintaining initial quality of semen, indicating estimation of these hormones as a valuable tool
fo~ _initial selection of bulls and _also for the selection of ejaculates for freezing.