Release of transaminases subsequent to preservation in diluents . containing EDTA and cysteine HCL of beetal and cross bred (Beetal x Black bengal) buck spematozoa

Abstract

The study was conducted to observe the role of EDT A and Cysteine HCL on the semen of buck
spermatozoa during preservation at refrigerator temperature. Egg Yolk citrate and Tris egg yolk fructose citric acid
and yolk with EDTA and Cysteine HCL added@ 1 mg/ml in each extender separately were used for preservation.
The leakage of transaminases (AST and ALT) from the spermatozoa at different hours of preservation in different
extenders was observed. Results obtained during the present study indicate that leakage of both AST and ALT enzymes was reduced by inclusion of Cysteine HCL and EDT A in both the diluents as compared to control (EYC
and TEYFC) extender at all hours of preservation.