Bacterial flora in frozen semen of Murrah buffalo bulls and their antibiogram

Abstract

A total of 28 frozen semen samples were screened for bacteria and 29 bacterial isolates were obtained.
Out of29 isolated bacteria, 6 were identified as Staphylococcus aureus (20.68%), 4 as Bacillus sp. (13.79%), 4 as
Escherichia coli (13.790/o), 4 as Alcaligenes faecalis (13.79%), 4 as Proteus sp. (13.79%), 4 as Citrobacter sp.
(13.790/o) and 3 as Pseudomonas aeruginosa (10,34%). Antibiotic sensitivity test was performed against 12
different antibiotic discs (Amikadn, enrofloxacin, gentamicin, chloramphenicol, ciprofloxacin, cephalexin,
streptomycin, amoxycillin, ampicillin, erythromycin, norfloxacin and penicillin). The results of sensitivity test
revealed that gram positive bacteria were more .susceptible as compared to gram negative bacteria with
antimicrobials used in the study. Bacillus sp. and Alcaligenes faeca/is were found to be susceptible to all the
antimicrobials used, whereas Pseuodomonas aeruginosa was sensitive to ciprofloxacin, gentamicin, amikacin and
enrofloxacin only. Amikacin and ciprofloxacin were found to be most effective (89.65%) against the isolates,
followed by enrofloxacin (86.20%), genatamicin (82.75%), norfloxacin (79.31%), chloramphenicol (72.41%),
cephalexin (68.97%) ampicillin (48.27%), streptomycin (44.82%) penicillin (37.93%), amoxycillin (31.03%) and
erythromycin (20.68%).