ANTISPERM ANTIBODIES IN SERUM AND CERVICAL MUCUS OF NORMAL AND REPEAT BREEDING COWS
Keywords:
Cross bred cows, Repeat breeding, Anti sperm antibodies, Serum and Cervical mucusAbstract
Indirect ELISA was performed to determine the antisperm-antibodies (asp-abs) in serum and
cervical mucus from 14 normal and 53 repeat breeding crossbred cows .85.71% sera and 78.57%
cervical mucus from normal breeding cows showed asp-abs titre of 1 :160 and 1 :40, respectively .On
the other hand 100% cows repeating 3-5 times or more showed the presence of asp-abs in serum
and mucus with the titre of 1 :3120 and 1: 1280, respectively. The significant observations made under
this study were that although a good proportion of normal cyclic cows were positive for asp-abs but
with lower level of titer in serum and mucus. The cows having more unsuccessful inseminations
showed higher asp -abs with high titre in serum and mucus.