CIRCULATING OVARIAN STEROID PROFILES IN SURTI BUFFALOES SUPEROVULATED WITH FSH AND DIFFERENT DOSES OF PMSG

Abstract

Eighteen Surti buffaloes of 2 to 6 lactations were superovulated during breeding season (August
to February) using FSH and 2 doses of PMSG. Animals were divided into three groups. Group I (n=6)
and Group II (n=6) donors were superovulated with single Vm dose of 2500 and 3000 IU PMSG,
respectively, on day 10 of the estrous cycle, whereas Group Ill (n=6) donors were treated intramuscularly
with 400 mg FSH per donor in 8 equal doses 12 hours apart for four days. Jugular samples were
collected from all donors at five different stages of MOET, i.e, Day O (Heat), Day 1 O (PMSG treatment),
Day 12 (2nd PGF 24 inj. ), Day 14 (Superovulatory estrus) and Day 20 (Flushing} for serum separation. No
significant variation was found in serum concentrations of progesterone and estradiol-171! among
three groups of donors during various stages of MOET. However, the serum concentrations of progesterone and estradiol-17B at various stages of MOET within the group varied significantly (p<0.05). The number of Cls had significant positive correlations with the serum progesterone (r = 0.125) and
estradiol-17B (r = 0.482) profile on pooled basis. The mean serum concentrations of progesterone .
were found to be highest on the day of embryo collection (1.83 ± 0.63; 3.01 ± 0.23 and 3.35 ±0.68 ng/ ml) in 3 groups (Group I, II and 111, respectively) of donors, whereas the mean serum concentrations of
estradiol-17B were found to be the highest on the day of superovulatory estrus (42.67 ± 5.44; 46.17 ±
6.27 and 50.17 ± 7 .12 pg/ml, respectively).