EFFECT OF CYSTEAMINE SUPPLEMENTATION IN SEMI DEFINED MEDIA ON IN VITRO PRODUCTION OF BUFFALO EMBRYOS

Authors

  • A PALANISAMY Centralized Embryo Biotechnology Unit, Department of Animal Biotechnology, Madras Veterinary College, Chennai - 600 007.
  • S RANGASAMY
  • S SATHESHKUMAR
  • K KUMANAN

Keywords:

Buffalo, Cysteamine, In vitro fertilization, Embryo

Abstract

The present study was conducted to investigate the effect of cysteamine supplementation in semi defined media like synthetic oviductal fluid (SOF) media during various stages of in vitro culture on buffalo preimplantation embryo development. Six independent trials with a total of one hundred and
seventy four oocytes were carried out with cysteamine supplementation at 1 00μM/ml during in vitro maturation (IVM), fertilization (IVF) and culture (IVG), one hundred twenty six oocytes without supplementation served as control. The mean cleavage rates were 24.1 ±1.67and 19.8±0.89 per cent in
the treatment and control group, respectively. The percentage of morula that developed in treatment and
control group were 15.1 ±1.35 and 7.9±1.47, respectively. The percentage of embryo that developed to
the morula stage were significantly higher (P<0.01) in treatment group. Cysteamine supplementation during IVM, IVF and IVG improved the cleavage and morula development due to glutathione (GSH) synthesis stimulated by cysteamine.

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Published

2023-01-23

Issue

Vol. 30 No. 2 (2009): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

PALANISAMY, A., RANGASAMY, S., SATHESHKUMAR, S., & KUMANAN, K. (2023). EFFECT OF CYSTEAMINE SUPPLEMENTATION IN SEMI DEFINED MEDIA ON IN VITRO PRODUCTION OF BUFFALO EMBRYOS. The Indian Journal of Animal Reproduction, 30(2), 36–38. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4880