STUDIES ON IN-VITRO CULTURING OF BUFFALO (BUBALUS BUBALIS) FETAL FIBROBLAST STEM CELLS

Authors

  • P ANJANA Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
  • K KUMANAN
  • M PARTHIBAN
  • K VIJAYARANI
  • S BALASUBRAMANIAM

Keywords:

Stem cells, Buffalo fetal fibroblast stem cells

Abstract

The present study describes the isolation, in-vitro culturing and characterization of fetal stem cells from
buffalo (Bubalus bubalis). During early stages of culture, buffalo fetal fibroblast (BFF) cells had heterogeneous
morphology which developed into uniform fibroblast morphology lafer. Single cell derived BFF stem cell (BFFSC)
clones were assessed for their osteogenic, chondrogenic and adipogenic potentials using specific differentiation
medium. BFFSCs differentiated into osteogenic, chondrogenic and adipogenic lineages as observed by special
staining. Total RNA was isolated from BFFSCs and checked for the presence of the three stem cell markers
namely, Oct-4, Sox-2 and Nanog, and all three transcription factor genes were detected in differentiated BFFSCs.

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Published

2023-01-20

Issue

Vol. 32 No. 1 (2011): Indian Journal of Animal Reproduction (Jan-jun)

Section

Research Article

How to Cite

ANJANA, P., KUMANAN, K., PARTHIBAN, M., VIJAYARANI, K., & BALASUBRAMANIAM, S. (2023). STUDIES ON IN-VITRO CULTURING OF BUFFALO (BUBALUS BUBALIS) FETAL FIBROBLAST STEM CELLS. The Indian Journal of Animal Reproduction, 32(1), 31–34. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4818