IN-VITRO CAPACITATION OF BOVINE SPERMATOZOA BY MEASURING PENETRATING POTENTIAL

Authors

  • S SINGH Department of Animal Reproduction, Gynaecology and Obstetrics . College of Veterinary and Animal Sciences GB.Pant University of Agriculture and Technology Pantnagar-263 145, U.S.Nagar (Uttarakhand)
  • H P GUPTA
  • A K MISHRA
  • A KUMAR

Keywords:

Buffalo spermatozoa, Caffeine, Capacitation, Gel penetration

Abstract

Penetrating potential of bovine spermatozoa, capacitated in vitro, w~ studied in terms of sperm penetratio
distance (SPD) to determine the suitable combination of caffeine concentration and incubation period foroptimu~
capacitation. The capacitation was induced in sperm TALP (Tyrode'S albumin, lactate and pyruvate) medium
containing 0, 5, 10 and 20 mM caffeine at 39 °C for 0, 30, 60 and 90 min. The c~pacitated spermatozoa were
subjected to penetration in 2% polyacrylamide gel and SPD was measured. The maximum SPD of 19.35±0.50
mm /hr was recorded at 20mM caffeine with 90 min incubation period indicating this period and concentration
combination as the most effective enhancer of in vitro capacitation of bovine spermatozoa.

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Published

2023-01-18

Issue

Vol. 32 No. 1 (2011): Indian Journal of Animal Reproduction (Jan-jun)

Section

Research Article

How to Cite

SINGH, S., GUPTA, H.P., MISHRA, A.K., & KUMAR, A. (2023). IN-VITRO CAPACITATION OF BOVINE SPERMATOZOA BY MEASURING PENETRATING POTENTIAL. The Indian Journal of Animal Reproduction, 32(1), 4–6. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4737