DETECTION OF BUFFALO PREGNANCY ASSOCIATED GLYCOPROTEIN-1 GENE TRANSCRIPTS AT DIFFERENT STAGES OF PREGNANCY

Abstract

Pregnancy is established and maintained in response to a series of interactions between the conceptus and
maternal reproductive tract through a large family trophoctoderm proteins i.e. Pregnancy associated glycoprotein.
PAG-1 gene has been characterized and its expression has been detected in many domestic, wild ruminants as
well as pigs but not in buffalo. In this study buffalo PAG-1 gene transcripts was PCR amplified during various
stages of pregnancy i.e. (Day 30, 50, 60, 70, 90, 110, 150 and full term). Moreover buffalo PAG-1 gene was
characterized using EcoR !: as restriction enzyme showed the presence of the conserved site for EcoR I for every
binucleate specific PA Gs especially PAG-1. From the present study, it was found that buffalo Pregnancy Associated
Glycoprotein-1 gene was expressed throughout gestation which could be detected qualitatively confirming the
expression of the buffalo Pregnancy Associated Glycoprotein-1 gene transcripts throughout pregnancy similar to
cattle and other species.