EFFICACY OF THE TRIS AND BIOCIPHOS PLUS EXTENDERS ON THE FREEZABILITY OF PUNGANUR BULL SEMEN
Keywords:
Punganur bulls, Semen, Freezability, Tris buffer, Biociphos PlusAbstract
The present investigation was carried out to study the freezability of Punganur bull semen with Tris and
Biociphos plus extenders. The difference in the individual sperm motility and live sperms between fresh and
Biociphos plus extended semen and between Tris and Biociphos plus extended semen during pre-freeze and
post-thaw periods was significant. The difference in head, mid- piece, tail and total sperm abnormalities between
fresh and Tris extended semen was not significantly different but between Tris and Biociphos plus extended
semen during pre-freeze and post-thaw periods was significant. Both the extenders were relatively non-toxic to
sperm, but based on the sperm motility, livability, sperm abnormalities and acrosome damage Tris extender was
concluded a better choice for preservation of Punganur bull semen.