SUPEROVULATORY RESPONSE AND EMBRYO RECOVERY IN SURTI BUFFALOES USING FSH AND DIFFERENT DOSES OF PMSG

Abstract

The experiment was conducted to study superovulatory response and embryo recovery rate in
18 Surti buffaloes of 2-6 parity during breeding season (August to February) using FSH and two doses
of PMSG. Animals were divided into three groups. Group I (n=6) and Group II (n=6) donors were
superovulated with single i/m dose of 2500 and 3000 I U PMSG, respectively, on day 10 of the estrous
cycle, whereas Group Ill (n=6) donors were treated intramuscularly with 400 mg FSH per donor in 8
equal doses 12 hrs apart. Luteolysis was carried out on day 12 using PGF2«. A!l the animals were bred
at _superovulatory estrus. Almost all the animals responded to superovulation. Flushing was performed
on day 6 after superestrus. The mean number of ovulations and embryo recovery rates in three groups
(I, II and Ill) were2.67, 3.50, 4.67 and 0.00, 0.17, 0.17, respectively. The mean numbers of unovulated
follicles were 1.67, 2.17 and 1.00, respectively, in the three groups.