COMPARISON OF TCM-199 AND SOF MEDIA ON IN VITRO DEVELOPMENT OF OVINE PREIMPLANTATION EMBRYOS

Authors

  • S RANGASAMY Centralized Embryo Biotechnology Unit, Department of Animal Biotechnology, Madras Veterinary College, Chennai - 600 007.
  • A PALANISAMY
  • S SATHESHKUMAR
  • CH SRINIVASA PRASAD
  • A MAHALINGA NAINAR

Keywords:

ln vitro development, Ovine oocytes, TCM-199, SOF media

Abstract

The aim of the present study was to compare the effectiveness of two different media viz undefined
media (TCM-199) and semi defined (SOF) media for in vitro development of ovine preimplantation
embryos. A total of 17 4 ovaries were sliced and 782 oocytes were recovered with a total recovery rate
of 4.5 oocytes per ovary and cultureable oocytes recovery rate of 2. 7 oocytes per ovary. The presumptive
zygotes derived from in vitro maturation and fertilization (IVM/IVF) were randomly subjected for two in
vitro culture (IVC) systems (TCM-199 and SOF), Statistical analysis revealed that highly significant
(P>0.01) percentage of embryos reached morula after cleavage in SOF media (30.4±1 .14) than TCM~·-.
199 (21.7±0.93). From this study, it was concluded that semi defined media like SOF support high
rate of ovine morula development than undefined media like TCM-199 complex media due to amino
acids included in SOF media and a biphasic effect of bovine calf serum inhibiting the first cleavage and
promoting further morula and blastocyst development in TCM-199 media.

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Published

2023-01-17

Issue

Vol. 31 No. 1 (2010): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

RANGASAMY, S., PALANISAMY, A., SATHESHKUMAR, S., PRASAD, C.S., & NAINAR, A.M. (2023). COMPARISON OF TCM-199 AND SOF MEDIA ON IN VITRO DEVELOPMENT OF OVINE PREIMPLANTATION EMBRYOS. The Indian Journal of Animal Reproduction, 31(1), 10–12. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4517