EFFECT OF FERTILITY ASSOCIATED PROTEIN ON OXIDATIVE ,nR1-1 STRESS OF BOVINE SPERM CELLS

Authors

  • M KARUNAKARAN Department of Animal Reproduction, Gynaecology and Obstetrics, j El- Madras Veterinary College, TANUVAS, Chennai- 600 007
  • T G DEVANATHAN
  • K KULASEKAR
  • P SRIDEVl
  • TILAK PON JAWAHAR
  • K LOGANATAHSAMY
  • A DHALI
  • S SELVARAJU

Keywords:

Bull, Semen, Oxidative stress, Lipid peroxidation, Malondialdehyde, Fertility associated protein

Abstract

The present study addressed the effects of fertility associated protein on oxidative stress in frozen thawed bull
spermatozoa. The frozen semen samples of 22 bulls were thawed at 37° C in a water bath and oxidative stress
was measured indirectly by estimating malondialdehyde (MDA) level at immediate post thaw, 60, 120 and 180
min of incubation. To find whether addition of fertility associated protein would control the oxidative stress of the
sperm cells, 25 μg of 28 - 30 kDa heparin binding protein of the sperm membrane was added to the thawed semen
samples and MDA levels were estimated. MDA level was significantly lower (P < 0.01) in fertility associated
protein treated semen sample than the untreated control semen at 60 min (1.95 ± 0.11 vs 2.85 ± 0.12), 120 min
(2.40 ± 0.12 vs 3.20 ± 0.20) and 180 of incubation (2.99 ± 0.15 vs 4.31 ± 0.32). It was concluded that 28- 30 kDa
heparin binding protein of the sperm membrane could help in controlling the oxidative stress of the sperm cells
during incubation.

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Published

2023-01-16

Issue

Vol. 33 No. 1 (2012): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

KARUNAKARAN, M., DEVANATHAN, T.G., KULASEKAR, K., SRIDEVl, P., JAWAHAR, T.P., LOGANATAHSAMY, K., … SELVARAJU, S. (2023). EFFECT OF FERTILITY ASSOCIATED PROTEIN ON OXIDATIVE ,nR1-1 STRESS OF BOVINE SPERM CELLS. The Indian Journal of Animal Reproduction, 33(1), 43–46. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4457