EFFICACY OF IN-VITRO MATURATION OF BUBALINE OOCYTES IN TCM 199, TCM 199 + PMSG AND FOLLICULAR FLUID

Abstract

A total number of 498 ovaries were procured from municipal slaughter house, Hyderabad. The ovaries were
subjected to slicing, aspiration and post aspiration slicing in order to retrieve the oocytes. The oocyte retrieval rate
was 73.68, 43.61 and 47.91 per cent in slicing , aspiration and post aspiration slicing methods, respectively. The
oocyte recovery per ovary was 2.91, 1.53 and 1.89 in slicing, aspiration and post aspiration slicing, respectively.
Retrieved oocytes were graded into A, B, C and D based on cellular investment and homogeneity. The yield of
grade A oocytes were 53.92, 42.57 and 46.72 in slicing, aspiration and post aspiration slicing techniques,
respectively. The yield of grade B oocytes was 28.87, 20.92 and 29.10, respectively. The yield of grade C and D
oocytes were 15.69 and 9.52; 11.27 and 11 .24; and 13.93 and 10.25 in slicing, aspiration and post aspiration
slicing methods, respectively. The A, B and C graded oocytes were matured in TCM 199, buffalo follicular fluid
(bu FF) alone and TCM 199 supplemented with PMSG. Based on the degree of cumulus expansion, the maturation
was classified as DO, D1 and D2 cumulus expansion. Significantly higher per cent of grade A and B oocytes
attained D2 and D1 degree of cumulus expansion while significantly higher per cent of C grade oocytes retained in
DO degree of cumulus expansion. The per cent of grade A oocytes extruded first polar body was 70.83, 5.55 and
42.50 in PMSG, bu FF and TCM 199 media, respectively Higher per cent of oocytes could be harvested by post
aspiration and slicing technique and commercially available PMSG could be supplemented to TCM 199 media for
invitro-maturation of follicular oocytes.