ASSESSMENT OF ACROSOMAL INTEGRITY OF DOG SPERMATOZOA USING SPERMAC STAINING TECHNIQUE

Authors

  • J UMAMAGESWARl Department of Animal Reproduction, Gynaecology and Obstetrics Madras Veterinary College, Chennai - 600 007
  • CECILIA JOSEPH
  • K KULASEKAR
  • J KALATHARAN
  • P SRIDEVl

Keywords:

Acrosome, Sperrnac stain, Programmable freezing

Abstract

Semen was collected by digital manipulation from eighteen matured dogs of three different breeds. The
sperm rich second fraction of semen samples were diluted, cooled and frozen in programmable freezer at three
different cooling rates. In an attempt to monitor any changes occurring to the acrosome during freezing, smears
made with fresh semen and with frozen semen at various cooling rates were stained with spermac stain and
examined under 400x magnifications. Most acrosomal damage appeared to occur during fast and medium
cooling rates than in slow cooling rate of programmable freezing. Further, the use of Spermac stain with slight
modification in staining technique in the present study provides simple and accurate assessment of the acrosomal
membrane changes in frozen thawed dog spermatozoa.

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Published

2023-01-14

Issue

Vol. 33 No. 2 (2012): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

UMAMAGESWARl, J., JOSEPH, C., KULASEKAR, K., KALATHARAN, J., & SRIDEVl, P. (2023). ASSESSMENT OF ACROSOMAL INTEGRITY OF DOG SPERMATOZOA USING SPERMAC STAINING TECHNIQUE. The Indian Journal of Animal Reproduction, 33(2), 51–53. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4357