SEASONAL VARIATION IN SEMINAL BIOCHEMICAL CONSTITUENTS IN SURTI BUFFALO BULLS

Abstract

This investigation was carried out using 60 semen ejaculates (10 ejaculates/bull/season) obtained from 2
sexually mature healthy Surti buffalo bulls over one year period. The bulls were managed identically and were
under weekly twice semen collection schedule in AV. The year was divided in to three seasons (monsoon, winter
and summer).Each ejaculate was divided into two aliquots; one was assessed for physico-morphological and
functional attributes, while other was used for evaluation of seminal biochemical and enzymatic profile. The overall
mean initial seminal fructose content was 586.61±65.77 mg/di. The average seminal plasma contents of total
protein, total cholesterol and enzyme ALP, ACP, AST andAL T activity recorded were2.54 ± 0.51 g/dl, 53. 71±10.05
mg/di, 53.71±10.05 KAU/dl, 246.78 ± 27.92 KAU/dl, 57.30 ± 10.73 IU/L and 15.38 ± 5.75 IU/L, respectively.
Seasonal influence was highly significant (P<0.01) for initial fructose and all four enzymes. The lowest values for
fructose, protein, ALP and ALT were observed during summer and for cholesterol, ACP and ALT during monsoon.
The bulls varied significantly (P<0.01) only in their seminal fructose, protein and ALP. Among the seminal profile,
significant correlations were observed for fructose with AST (-0 .43 ), ALT (0. 36) and AC P (-0. 38); total protein with
AST (-0.76); cholesterol withACP (0.27); AST with ALT (-0.43), ALP (-0.44) and ACP (0.46); ALT with ALP (0.42),
and ALP with ACP (-0.41 ). Further, significant correlations were found for ejaculate volume with seminal fructose
(0 .46) and ACP (-0. 35 );sperm concentration with seminal ALP (-0 .54 ); mass activity with seminal fructose (-0 .29),
AST (0.44), ALT (~0.28), ALP (-0.39) and ACP (0.26):seminal pH with cholesterol (0.26):live sperm with seminal
ALP (-0.41); abnormal sperm with seminal fructose (0.33), cholesterol (-0.41 ),AST (-0.32), ALP (0.27) andACP (-
0.51 ); intact acrosome with seminal ALT (-0.33); HOS reactive sperm with seminalAST (0.32); cold shock resistant
sperm with seminal fructose (0.34),AST(-0.36), ALT (0.29), ALP (0.59) andACP (-0.37) and MB RT with seminal
fructose (0.32).