EFFECTS OF DIFFERENT COOLING RATES USING PROGRAMMABLE FREEZER ON POST THAW SURVIVAL OF DOG SPERMATOZOA

Authors

  • J UMAMAGESWARl Department of Animal Reproduction, Gynaecology and Obstetrics Madras VeterinaryCo//ege, Chenna/-600001.
  • CECILIA JOSEPH
  • K KULASEKAR
  • J KALATHARAN
  • P SRIDEVl

Keywords:

Spermatozoa, Acrosomal integrity, Programmable freezing

Abstract

Semen collected by digital manipulation from eighteen healthy and sexually matured dogs of three different
breeds viz., Labrador Retriever (n=B), German Shepherd (n= 6), Boxer ( n = 4) aged between 2 to 6 years was
diluted with Tris - fructose - citric acid extender containing 8% glycerol and 20% egg yolk at the rate of 1 :2 and
packed in French mini straws. They were frozen at the three different cooling rates of programmable freezing viz.
Slow (R1), Medium (R2) and Fast (R3) at -1p C/min for 30min, -Sp C/min for 10min and -10p C/min for 7min
respectively. The mean percentages of post-thaw motility, live spermatozoa, normal spermatozoa, acrosomal
integrity and plasma membrane integrity were significantly higher (P.::. 0.05) following slow cooling rate (R 1) than
other rates of programmable freezing. Hence, slow cooling rate (R1) of programmable freezing is suggested to be
optimum for freezing dog semen.

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Published

2023-01-14

Issue

Vol. 33 No. 2 (2012): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

UMAMAGESWARl, J., JOSEPH, C., KULASEKAR, K., KALATHARAN, J., & SRIDEVl, P. (2023). EFFECTS OF DIFFERENT COOLING RATES USING PROGRAMMABLE FREEZER ON POST THAW SURVIVAL OF DOG SPERMATOZOA. The Indian Journal of Animal Reproduction, 33(2), 19–22. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4345