EFFECT OF SUPPLEMENTATION OF CAFFEINE ON SPERM MOTILITY AND LIVEABILITY IN MARWARI STALLIONS

Abstract

Six ejaculates from each three adult stallions were collected via artificial vagina using estrus jenny as dummy.
Caffeine was added to semen extender at 0.1, 0.5 and 2 mM during pre-freeze stage and cryopreserved by following
standard protocol. The sperm kinetic parameters, membrane functionality and integrity were evaluated with computerassisted
sperm analysis, a hypo-osmotic swelling test and epifluorescent microscopy, respectively. The present study
revealed that, adding caffeine to stallion semen extender at 0.1, 0.5 and 2 mM levels had positive impact on seminal
parameters compared to the control semen. Sperm kinetics (VAP, VSL and STR) and motility were increased in the
caffeine treated groups in comparison to the control group. Among these, 2mM caffeine supplemented group the
post-thaw sperm motility and other functional attributes have significantly improved compared to the other treatment
and control groups.