DUAL STAINING IDENTIFIES A GREATER PROPORTION OF MORIBUND SPERMATOZOA IN STALLION SEMEN WITH POOR CRYOTOLERANCE

Authors

  • NILENDU PAUL 1Theriogenology Laboratory, Southern Regional Station of ICAR-National Dairy Research Institute, Bengaluru – 560030, Karnataka, India
  • T R TALLURI
  • YASHPAL
  • R A LEGHA
  • PRADEEP NAG
  • ARUMUGAM KUMARESAN

Keywords:

Stallion, spermatozoa, motility, membrane integrity, dual staining

Abstract

The present study was conducted to evaluate the functional membrane integrity of cryopreserved spermatozoa
from stallions belonging to two contrasting motility groups (high and low motility groups). A dual staining method
comprising of carboxy fluoroscein diacetate (CFDA) and propidium iodide (PI) was used for this purpose. Stallions
were classified into either high (post thaw progressive motility e”35%) or low (post thaw progressive motility <25%)
motility groups. The proportion of membrane intact spermatozoa was significantly (P<0.05) higher in high motility
group. Interestingly, it was observed that the proportion of moribund population was significantly (P<0.05) higher in
low motility group as compared to high motility group. It was inferred that higher proportion of moribund spermatozoa
might be one of the important contributing factors for poor sperm motility in stallions belonging to low motile group.

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Published

2023-01-11

Issue

Vol. 41 No. 2 (2020): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

PAUL, N., TALLURI, T.R., YASHPAL, LEGHA, R.A., NAG, P., & KUMARESAN, A. (2023). DUAL STAINING IDENTIFIES A GREATER PROPORTION OF MORIBUND SPERMATOZOA IN STALLION SEMEN WITH POOR CRYOTOLERANCE. The Indian Journal of Animal Reproduction, 41(2), 28–31. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/4188