EFFECT OF E. COLI LPS AND LUGOL’S IODINE ON CONCEPTION RATE IN REPEAT BREEDER COWS WITH SUBCLINICAL ENDOMETRITIS

Abstract

Forty-five repeat breeder cows positive for subclinical endometritis by white side test were divided randomly into
three groups. Group-I cows (LPS) were administered 100 μg of E. coli LPS in 30 ml sterile phosphate buffered saline
intrauterine once at estrus. Group-II cows (LI) were administered 1% Lugol’s iodine in 30 ml of distilled water (LI).
Group-III cows (Control) were administered 30 ml sterile PBS intrauterine once at estrus. Haematological parameters
such as Hb, TLC and DLC were studied before initiation of treatment and on the subsequent estrus also. AI was done
in the subsequent estrus in all the cows. Pregnancy verification was done 45th day post AI. The mean Hb level
significantly increased in LPS and LI groups in subsequent estrus. The TLC count significantly decreased in LPS and
LI groups in subsequent estrus. There was significant increase in the mean lymphocyte count and significant decrease
in neutrophil count in LPS group in subsequent estrus. There was significant decrease in monocyte count in LPS,
control group and significant decrease in the eosinophil count in LI group in the subsequent estrus. The first service
conception rate was 53.33%, 20.00 % and zero per cent for LPS, LI and control groups, respectively.