EFFECT OF DIFFERENT GONADOTROPINON IN VITRO ON DEVELOPMENT OF IMMATURE CAPRINE OOCYTES

Abstract

The objective of the present study is to compare the embryo development from caprine oocytes matured
in vitro in a medium supplemented with PMSG alone or in combination with hCG. Cumulus oocyte complexes
(573) were matured in two treatment groups. Group 1 (n=377) COCs were matured inTCM-199 medium
supplemented with 20 IU/ml PMSG. Group 2 (n=196) COCs were matured inTCM-199 medium supplemented
with 20 IU/ml PMSG+20 IU/ml hCG. After 27h of maturation, oocytes were fertilized in mTALP and cultured
in mCR2aa medium. The maturation rate in groups 1 and 2 were 88.63% and 91.78% respectively. Similarly,
cleavage rates, morula and blastocyst production in groups 1 and 2 were 47 .13, 20.00, 10.00 and 43.21, 22.85,
7.14% respectively. The results indicated that oocytes matured with 20IU/ml PMSG had a good cytoplasmic
maturation that allows normal embryo development up to blastocyst stagewithout addition of hCG. Thus,
PMSG at 20IU/ml in maturation medium can be used for in vitro embryo production.