EFFECT OF DIFFERENT GONADOTROPINON IN VITRO ON DEVELOPMENT OF IMMATURE CAPRINE OOCYTES
Keywords:
Blastocyst, Cleavage, Goats, hCG, PMSGAbstract
The objective of the present study is to compare the embryo development from caprine oocytes matured
in vitro in a medium supplemented with PMSG alone or in combination with hCG. Cumulus oocyte complexes
(573) were matured in two treatment groups. Group 1 (n=377) COCs were matured inTCM-199 medium
supplemented with 20 IU/ml PMSG. Group 2 (n=196) COCs were matured inTCM-199 medium supplemented
with 20 IU/ml PMSG+20 IU/ml hCG. After 27h of maturation, oocytes were fertilized in mTALP and cultured
in mCR2aa medium. The maturation rate in groups 1 and 2 were 88.63% and 91.78% respectively. Similarly,
cleavage rates, morula and blastocyst production in groups 1 and 2 were 47 .13, 20.00, 10.00 and 43.21, 22.85,
7.14% respectively. The results indicated that oocytes matured with 20IU/ml PMSG had a good cytoplasmic
maturation that allows normal embryo development up to blastocyst stagewithout addition of hCG. Thus,
PMSG at 20IU/ml in maturation medium can be used for in vitro embryo production.
