HORMONAL AND IMMUNOLOGICAL STUDIES IN DIFFERENT TRIMESTER OF PREGNANCY IN COWS

Abstract

This investigation was carried out with an objective to study serum progesterone and total immunoglobulin
levels of cows in three different trimesters of pregnancy. A total of 10 pregnant cows were selected for the
investigation besides 10 normal, healthy and non-pregnant cows at oestrus which were considered as control
group. Blood sample collected in each gestational stage and serum sample were estimated through kits
supplied by Crest Biosystems. The serum progesterone values (ng/ml) observed in first, second and third
trimester of pregnancy were 4.85 ± 1.5, 5.76 ± 0.46 and 4.75 ± 1.36 respectively. The cows at oestrus recorded
a value of 0.88 ± 0.14 (ng/ml) for serum progesterone concentration. The analysis of variance revealed a
highly significant difference (p<0.01) among various stages of pregnancy and non-pregnant (control) group.
The total immunoglobulin concentrations in blood serum (g/dl} were estimated to be 2.65 ± 0.10, 3.20 ± 0.1 O,
and 2. 73 ± 0.06 for 1st, 2m1 and 3rd trimester of pregnancy respectively whereas in non-pregnant control group
the value was 2.71 ± 0.07 g/dl. Analysis of variance revealed a significant difference (p<0.01) among various
trimester of pregnancy.