EFFECT OF DIFFERENT CRYOPROTECTANTS ON SPERM PLASMA MEMBRANE INTEGRITY OF OSMANABADI AND SIROHI BUCK SEMEN

Abstract

Semen was collected from six Osmanabadi and six Sirohi bucks weekly for six weeks by Artificial Vagina
method. Semen was diluted using Tris Egg yolk Glycerol (TYG), Tris Egg yolk Ethylene Glycol (TYE) and Tris
-Dimethyl sulphoxide (DMSO) dilutors (TYO) and evaluated for sperm plasma membrane integrity by HOS test
at pre (S°C) and post freezing (-196°C at 24 and 72 hours) stage. At pre freezing (S°C) stage in Osmanabadi
buck semen there was no significant difference (P>0.0S) across different dilutors with respect to HOS positive
sperm percentage but in Sirohi bucks significantly higher sperm plasma membrane integrity was observed in
TYO and TYG than TYE diluter. At 24 hours post freezing stage in Osmanabadi buck semen significantly higher
plasma membrane integrity was noted in TYO than TYG am.I TYE dilutors. In Sirohi buck ::;ernen significantly
higher plasma membrane integrity was noted in TYO than TYE diluter, TYG being intermediate. At 72 hrs post
freezing stage in Osmanabadi bucks semen there was no significant difference (p>0.0S) across different dilutors
with respect to HOS positive sperm percentage. In Sirohi bucks semen significantly, higher (P<0.0S) percentage
of plasma membrane integrity was observed in TYO than TYE dilutors. It was concluded that in both the breeds
higher plasma membrane integrity was observed in TYO diluter which indicates that DMSO was more effective
cryoprotectant than ethylene glycol and glycerol in preserving sperm membrane integrity during cryopreservation of
Osmanabadi and Sirohi bucks semen.