ULTRASONOGRAPHIC ELECTIVE SUPEROVULATION AND ITS RESPONSES IN CROSSBRED AND ZEBU CATTLE

Abstract

In order to improve the total and viable embryo recovery rates, ultrasonography was used in cattle before
induction of superovulation (SOV). The conventional SOV protocol followed in crossbred and indigenous donors at
Sabarmati Ashe ram Gaushala was complemented by use of ultrasonography for selection of donors a day before
stimulation. Donors with minimum 5 growing follicles were used for SOV. Dominant follicle (DF) if any was ablated
24 hours before SOVtreatment. Total 54 donors were programmed from April 2010 to April 2011, out of which 49
were flushed with recovery of 362 (7 .39) total embryos and 219 (4.47) viable embryo from 500 (10.20) ovulations;
followed by 27 pregnancies from 114 embryo transfers. Data was analyzed for cross bred group (ablation vs non
ablation) and indigenous group (ablation vs non ablation). Crossbred group with ablation showed highest average
12.26 ± 1.49 (mean± SE) ovulations, 11.29 ± 1.66 total embryos and 6.18 ± 1.20 viable embryos. Indigenous
group with ablation had an average of 10.13 ± 1.62 ovulations, 6.38 ± 1.59 total embryos and 5.63 ± 1.24 viable
embryos. Selection of donor using ultrasonograpghy and selective ablation of dominant follicle improved the SOV
responses.