EFFECT OF INSULIN ADMINISTRATION DURING LUTEAL PHASE ON PROGESTERONE PROFILE AND CONCEPTION RATE IN BUFFALOES (BUBALUS BUBALIS)
Keywords:
Buffalo, Insulin, Progesterone, Conception rateAbstract
The present study was conducted to test the effect of insulin administration (Human Mixtard®, S/C@ 0.25
IU/kg body weight} during luteal phase on serum progesterone profile and conception rate in buffaloes. Estrus was
induced using 2ml (263μg/ml) cloprostenol sodium (im). Buffaloes were bred naturally during estrus. Insulin was
injected on day 10 of estrous cycle in treatment group (n=10), while PBS was given s/c to the control group (n=1 O)
on same day. The conception rate was higher in the treatment group (70%) than the control group (50%}. The
serum progesterone concentration remained higher from day 12 to 30 in the treatment group than the control group
and in pregnant than non-pregnant animals. Thus, it may be concluded that the insulin administration during luteal
phase had beneficial effect on the serum progesterone concentration and conception rate.