EFFECT OF CYSTEIN HYDROCHLORIDE AND VITAMIN E SUPPLEMENTATION ON QUALITY OF CRYOPRESERVED HARIANA BULL SEMEN

Abstract

The present study was aimed to evaluate the beneficial effects of supplementation of cystein hydrochloride and
Vitamin E on seminal attributes during different stages of semen cryopreservation in Hariana bull. Thirty two ejaculates
from four Hariana bulls were collected after initial examination the qualified ejaculates were divided into three aliquots;
one aliquot was extended with glycerolated egg yolk tris citrate (GEYT) extender (Control), second aliquot was extended
with GEYT supplemented with Cystein hydrochloride @ 5.0 mM (T1) and the third aliquot was extended with GEYT
supplemented with VitaminE @ 2.5 mM (T2). All semen samples were equilibrated and cryopreserved. The effect of
cystein hydrochloride and vitamin E was determined by assessment of sperm viability, progressive motility, hypoosmotic
response, and acrosomal integrity at three stages i.e. after dilution, pre-freeze and post –thaw. It was observed
thatsupplementation of cysteine hydrochloride (5mM) and vitamin-E (2.5 mM) caused significant (P<0.05) increase in
all sperm attributes at post thaw semen.