EFFECT OF RETRIEVAL TECHNIQUES ON THE QUALITY OF ABATTOIR OVARIAN FOLLICULAR OOCYTES IN ASSAM HILL GOATS

Authors

  • D BORAH Department of Animal Reproduction, Gynaecology and Obstetrics, Assam Agricultural University, Khanapara - 781 022
  • RK BISWAS
  • BC DEKA
  • S SINHA
  • L BARKALITA
  • RS BORAH
  • KC NATH
  • MK KALITA
  • RK DEWRY

Keywords:

Abattoir, Goat, Oocyte, Recovery rate, Slicing

Abstract

The oocytes recovered (n=2539) from the abattoir collected goat ovaries (n=712) by aspiration, slicing and puncture techniques were washed 4-5 times in a washing medium (36 ml TCM-199, 4 ml Foetal Bovine Serum and 200 μl Gentamicin) and were graded as A, B and C on the basis of their cumulus cell layers surrounding the zona pellucida. The mean recovery rate of oocytes per ovary using slicing technique was higher (4.21±0.08, p<0.05) compared to aspiration (3.43±0.06) and puncture (3.24±0.08) techniques. The recovery of good quality oocytes (Grade A+B) was higher (p<0.05) in puncture (73.9±0.9%) than in aspiration (66.3±0.7%) and slicing (64.8±0.9%) technique. It can be concluded that puncture technique was most effective for the retrieval of good quality goat oocytes, whereas, the slicing technique yielded highest oocyte recovery rate.

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Published

2022-12-31

Issue

Vol. 39 No. 2 (2018): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

BORAH, D., BISWAS, R., DEKA, B., SINHA, S., BARKALITA, L., BORAH, R., … DEWRY, R. (2022). EFFECT OF RETRIEVAL TECHNIQUES ON THE QUALITY OF ABATTOIR OVARIAN FOLLICULAR OOCYTES IN ASSAM HILL GOATS . The Indian Journal of Animal Reproduction, 39(2), 29–31. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3842