SEMINAL PLASMA REMOVAL IMPROVES CRYOPRESERVED SEMEN QUALITY IN GADDI BUCKS

Authors

  • A SHARMA Department of Veterinary Gynaecology and Obstetrics, CSKHPKV, Palampur -176062
  • P SOOD
  • PK DOGRA

Keywords:

Buck, Gaddi goats, Semen cryopreservation, Seminal plasma, Washing

Abstract

Forty-eight semen ejaculates, collected in artificial vagina, from eight adult Gaddi bucks were randomly divided into groups viz. freezing the entire semen sample (n=24) or removal of seminal plasma by centrifugation (n=24). Semen was extended in tris citrate egg yolk extender to maintain 150 million sperm / dose (0.25 ml). The filling, sealing, equilibration, vapour freezing and final plunging in liquid nitrogen was done as per the standard norms. The post-thaw results (%) of washed semen samples were better (p<0.05) than their non-washed counterparts (progressive motility 30.4±1.6 vs. 22.8±2.1; morphological abnormalities 9.7±0.9 vs. 14.3±0.8; HOST 61.5±2.0 vs. 45.6 ±3.8). In conclusion, the removal of seminal plasma in cryopreservation protocol of Gaddi buck semen appears essential to obtain better post-thaw results.

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Published

2022-12-31

Issue

Vol. 39 No. 2 (2018): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

SHARMA, A., SOOD, P., & DOGRA, P. (2022). SEMINAL PLASMA REMOVAL IMPROVES CRYOPRESERVED SEMEN QUALITY IN GADDI BUCKS . The Indian Journal of Animal Reproduction, 39(2), 25–28. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3841