EFFECT OF TWO DIFFERENT PERMEABLE CRYOPROTECTANTS ON FREEZABILITY OF EXOTIC STALLION AND JACK SEMEN

Authors

  • T R TALLURI Equine Production Campus, ICAR-National Research Centre on Equines, Bikaner
  • S K RAVI
  • C SINGH
  • R A LEGHA
  • s c MEHTA
  • V PRAKASH
  • B N TRIPATHI

Keywords:

Cryopreservation, Di-Methyl Formamide, Glycerol, Jack, Semen, Stallion

Abstract

Semen was collected from the exotic stallions and exotic jacks (n=5 each) using an artificial vagina. The
fresh semen was treated with primary extender and centrifuged to remove seminal plasma. Sperm pellet
obtained after centrifugation was equally divided to receive secondary extender containing same amount (5%)
of different permeable cryoprotectants, either glycerol (Gly) or dimethyl formamide (DMF) before submitting for
cryopreservation. Various sperm parameters were assessed at pre-freeze and post-thaw stage which revealed
differences (p<0.05) in motility, liveability, plasma membrane integrity and acrosome integrity of stallion as well as
Jack semen cryopreserved with DMF or Gly. A variation (p<0.05) existed between individual stallions and jacks
extended either with DMF or Gly. In brief, cryoprotectant DMF for stallion and Gly for jack semen were suitable for
retaining the plasma and acrosome integrity of spermatozoa.

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Published

2022-12-26

Issue

Vol. 40 No. 1 (2019): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

TALLURI, T.R., RAVI, S.K., SINGH, C., LEGHA, R.A., MEHTA, s c, PRAKASH, V., & TRIPATHI, B.N. (2022). EFFECT OF TWO DIFFERENT PERMEABLE CRYOPROTECTANTS ON FREEZABILITY OF EXOTIC STALLION AND JACK SEMEN. The Indian Journal of Animal Reproduction, 40(1), 27–30. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3767