EFFECT OF NON-GENETIC FACTORS ON THE QUALITY OF FROZEN SEMEN OF CROSSBRED HOLSTEIN FRIESIAN BULLS
Keywords:
Acrosome integrity, Frozen semen, Hypo-osmotic swelling, Non-genetic, Post-thaw motilityAbstract
Sixty five frozen semen samples of 14 crossbred Holstein Friesian bulls were subjected to quality assurance
tests such as post-thaw motility, post-thaw incubation survival, hypo-osmotic swelling (HOST) and acrosome
integrity. The data were statistically analyzed to study the effect of farm (I and II), ejaculate (I and II), period
(2007-2014) and season (summer, winter and northeast monsoon) on the frozen semen quality using general
linear model. Post-thaw motility and post-thaw incubation survival of spermatozoa was highly affected (p<0.01)
by ejaculate, period and season, whereas, HOST was affected by period, and acrosome integrity was affected by
ejaculate and period. In brief, non-genetic factors had impact on the quality of frozen semen of crossbred Holstein
Friesian bulls.