EVALUATION OF EFFECTIVE APPROACH FOR IN VITRO MATURATION OF CANINE OOCYTES WITH SEQUENTIAL ADDITION OF HORMONES

Authors

  • TR BABU Department of Veterinary Gynaecology and Obstetrics P.V. Narasimha Rao Telangana Veterinary University, Korutla - 505 326
  • KRC REDDY
  • KCS REDDY
  • VG KUMAR
  • R AMIN
  • G ARUNAKUMARI
  • A TEJA

Keywords:

Canine, COCs, In vitro maturation, Nuclear maturation, Oocytes

Abstract

About 134 canine ovaries were collected after routine ovariohysterectomy and 4,848 cumulus-oocyte complexes (COCs) were retrieved. Out of these, 1,080 good quality COCs were cultured in TCM-199 medium supplemented with 10% estrus bitch serum and incubated in 5% CO2 at 37оC for 96 h. These COCs were matured either without (control) or with sequential addition of gonadotropic and steroidal hormones. After 96 h maturation period, the percent COCs expansion and oocyte maturation rate was better in groups in which hormones were added in maturation medium.

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Published

2022-12-25

Issue

Vol. 38 No. 2 (2017): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

BABU, T., REDDY, K., REDDY, K., KUMAR, V., AMIN, R., ARUNAKUMARI, G., & TEJA, A. (2022). EVALUATION OF EFFECTIVE APPROACH FOR IN VITRO MATURATION OF CANINE OOCYTES WITH SEQUENTIAL ADDITION OF HORMONES. The Indian Journal of Animal Reproduction, 38(2), 24–27. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3718